Figure 5
From: Simultaneous Multiparameter Cellular Energy Metabolism Profiling of Small Populations of Cells
Formation of small cell colonies on the bottom substrate. (a) Process flow. First, to facilitate preferential adhesion to specific areas on the substrate, a solution of the extracellular matrix (ECM) protein fibronectin is deposited using a non-contact piezoelectric printing approach30; Second, cells suspended in culture medium are deposited and allowed to gravity-settle on the substrate; Third, the substrate is washed with PBS 3 times to remove the weakly adhered cells from the interstitial areas. (b) Fluorescence emission micrograph of cell colonies stained with JC-1 after 2 hours of incubation following deposition. The cell colonies are created in a 3 × 3 pattern matching that of the microwells on the top (lid) substrate of the device. A zoom-in on one of the colonies shows compactly positioned cells.
