Figure 5
From: ZFP226 is a novel artificial transcription factor for selective activation of tumor suppressor KIBRA
ZFP226 induced apoptosis in MCF-7 cells. Apoptosis of pZFP226-transfected MCF-7 cells was measured by Annexin-V/DAPI staining using flow cytometry. (A) Representative flow cytometry graphs showing the distribution of cells. Live cells (Q1 Annexin V−/DAPI−), early-apoptotic cells (Q2 Annexin V+/DAPI−), late-apoptotic/necrotic cells (Q3 Annexin V+/DAPI+) and cell debris (Q4 Annexin V−/DAPI+). (B) The number of apoptotic cells within quadrant Q2 and Q3 was significantly increased 48 hrs after ZFP226 transfection. (C) A significant increase for pro-apoptotic BAX and a significant decrease for anti-apoptotic BCL-2 was detected by real-time PCR 48 hrs after ZFP226 transfection. Flow cytometry and real-time PCR analysis are representative for experiments (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001.
