Figure 2 | Scientific Reports

Figure 2

From: Differential expression pattern of co-inhibitory molecules on CD4+ T cells in uncomplicated versus complicated malaria

Figure 2

Identification of differing cell signatures between children with uncomplicated and complicated malaria using automated hierarchical clustering. (A) CITRUS with group PAMR analysis (R implementation of Prediction Analysis for Microarrays) of PD-1, CTLA-4, LAG-3 and Tim-3 expression of samples from children with complicated (IP) and uncomplicated (OP) malaria. Differing clusters are highlighted in red (centre) and categorized into cluster group I (purple) and cluster group II (pink) (centre). Within each cluster group, clusters are numbered. Four parameter clustering of OP and IP samples demonstrating individual parameter expression intensities of PD-1, CTLA-4, LAG-3 and Tim-3 (outer graphs). Cv.1se was used with a 10 fold cross validation and error rate of 30%. (B) Each line represents a numbered cluster defined in A. Abundance of cells within the identified differing clusters in the OP and IP groups. The dots represent samples within the groups. (C) Intensity of the expression of co-inhibitory molecules within the differing clusters. Expression of the specific molecules within the total amount of cells is shown in grey, expression within the specific cluster is shown in red.

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