Table 2 List of primers used in this study.

a.

UPclpA Forward

5′ AAAAGCCTGAATGCAGGTATAAAAATTGGGGGAGGTGCCTTGTGTGTAGGCTGGAGCTGCCT 3′

To amplify kanamycin cassette with flanking regions of clpA

UPclpA Reverse

5′ CGGGCGCTAAGGCCCGGTTTGTACGACAGTGAAACGAAGACATATGAATATCCTCCTTA 3′

b.

UPclpB Forward

5′ TAATCTCCAGTAGCAATTTTGACCTGTTATGGGAGGAGTTTTGTGTAGGCTGGAGCTGCTTC 3′

To amplify kanamycin cassette with flanking regions of clpB

UPclpB Reverse

5′ AAACGAGCCCGTCAGGGCCCGTTTTATTCAAATTTGTGACCATATGAATATCCTCCTTA 3′

c.

clpA test deletion Forward

5′ AGAACGTGCAACGCAATTGATG 3′

To confirm the deletion of clpA from S. Typhimurium

clpA test deletion Reverse

5′ GGTTTGTACGACAGTGAAACGA 3′

d.

clpB test deletion Forward

5′ CTGGCGAATACCGGCGTT 3′

To confirm the deletion of clpB from S. Typhimurium

clpB test deletion Reverse

5′ ACAGACTTCTTAACGAAGCTTT 3′

e.

clpA amplification Forward

5′ ATATATGGATCCATGCTCAATCAAGAACTGGAAC 3′

To amplify and clone clpA in pQE60 (for complementation)

clpA amplification Reverse

5′ ATATATAAGCTTTTAGTGCGCGGCTTCCGG 3′

f.

clpB-pQE60_XhoI Forward

5′-ATATATCTCGAGATGCGTCTGGATCGTCTTAC-3′

To amplify and clone clpB in pQE60 (for complementation)

clpB-pQE60_BamHI Reverse

5′ATATATGGATCCTTACTGCACTGCCACAATAC-3′

g.

pQE60-clpB Forward

5′ATAAACAAATAGGGGTTCCGCG 3′

To confirm clpA-pQE60 clone

pQE60-clpB Reverse

5′ GGCGGCAACCGAGCGTTCT 3′

h.

pQE60-clpB Forward

5′ATAAACAAATAGGGGTTCCGCG 3′

To confirm clpB-pQE60 clone

pQE60-clpB Reverse

5′ GGCGGCAACCGAGCGTTCT 3′

i.

typh Forward

5′ TTGTTCACTTTTTACCCCTGAA 3′

S. Typhimurium specific primer. For confirmation of S. Typhimurium by amplifying typh gene

typh Reverse

5′ CCCTGACAGCCGTTAGATATT 3′

j.

clpA RT-PCR Forward

5′ ATGGCGCGTGTGATTCAGGAT 3′

To confirm expression of clpA by RT-PCR

clpA RT-PCR Reverse

5′ GGCTTCCGGCTTGTGCTTTTGC 3′

k.

clpB RT-PCR Forward

5′ CGGTTCCGATCTCATTCAGG 3′

To confirm expression of clpB by RT-PCR

clpB RT-PCR Reverse

5′ TGAGCAATAGAAGCGATGTGTT 3′