Figure 2
Sodium butyrate induces a dynamic and rapid increase in oxygen consumption rate in young fly whole head tissue. (A) Representative oxygen level changes showing decreased oxygen levels due to oxygen head consumption in control and SB treated samples. In SB treated fly heads, the oxygen drops to low levels and the OCR decreases at the last ‘ticks’. Therefore, we use the FIXED algorithm (See Methods). Each measurement (two minutes) consists of 10 sub-measurements referred to as a ‘tick’. The dashed line indicates the addition of SB. (B) Quantification of (A). Sodium butyrate (SB) induces a rapid and transient increase in oxygen consumption in the heads of young flies. A second injection after 10 measurements following the first injection results in a lower and shorter increase of OCR. Data was normalized to the measurement prior to addition of SB. The dashed lines indicate the addition of SB. N = 9 vehicle and 8 SB. (C) The addition of rotenone, a complex I inhibitor, significantly reduced OCR in whole fly head tissue. However, the addition of sodium butyrate induces increased OCR in the rotenone treated samples. The dashed line indicates the addition of rotenone or rotenone + SB. N = 9 per group. (*P < 0.05, **P < 0.01, ***P < 0.001). Error bars indicate the S.E.M. in all the graphs.
