Figure 4
TSA causes a dynamic and very rapid increase in oxygen consumption rate in young fly whole head tissue. (A) Representative oxygen head consumption in control and TSA treated samples. Similarly to the results in Fig. 2A, in TSA treated fly heads, the oxygen drops to low levels and the OCR decreases at the last ‘ticks’. We use the FIXED algorithm to model the results (See Methods). The dashed line indicates the addition of TSA. (B) Quantification of (A). TSA induces a very rapid and transient increase in oxygen consumption in the heads of young flies. A second injection after 10 measurements following the first injection results in a shorter increase of OCR but not lower. Data was normalized to the measurement prior to addition of TSA. The dashed lines indicate the addition of TSA. N = 5 vehicle and 6 TSA. (*P < 0.05, **P < 0.01). Error bars indicate the S.E.M. in all the graphs.
