Figure 3
From: Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases
The interaction between Itch and herpesvirus-encoding membrane proteins. HEK293T cells transfected with the plasmids of FLAG-ItchCA and HA-tagged viral adaptor proteins were analyzed by immunoprecipitation assay. Input fractions and immunocomplexes with anti-HA antibody were separated by SDS-PAGE and analyzed by Western blotting with the indicated antibodies (cropped, full-length blots are given in Supplementary Fig. S2). The IgG heavy chain (HC) and light chain (LC) are indicated.
