Figure 5
From: Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases
The induction of Itch ubiquitination by viral adaptor proteins. HEK293T cells were co-transfected with the plasmids of FLAG-ItchCA, myc-Ub and HA-tagged viral adaptor proteins in the indicated combinations. After 24 h post-transfection, cells were lysed with RIPA buffer and the lysates were subjected to immunoprecipitation assay with anti-FLAG antibody. Input fractions and precipitated FLAG-Itch CA proteins were analyzed by Western blotting with the indicated antibodies (cropped, full-length blots are given in Supplementary Fig. S3).
