Figure 1
From: Evaluation of a Miniaturized Biologically Vascularized Scaffold in vitro and in vivo
Qualitative and quantitative characterization of the native and acellular rat jejunal segment by histological and biochemical analysis. Segments of rat intestine were cannulated, explanted and subsequently decellularized. (a) Macroscopic appearance of the cannulated jejunal segment is shown right after explantation (a) and after decellularization (a’). (b–e) Histological analysis of the native rat jejunal segment (b–e) in comparison to the decellularized mBioVaSc (b’–e’): HE overview staining (b,b’); Feulgen reaction denoting DNA residues (c,c’); Masson’s Trichrome staining revealing residual collagen and ECM components (d,d’); Elastica van Gieson reaction showing elastic fibers within the matrix (e,e’). Scale bar, 100 μm. (f) Gelectrophoretical separation of DNA fragments before and after decellularization. (g) Quantitative determination of the DNA content in the native and decellularized tissue. (h,i) Quantification of the major ECM components: Collagen (h), and Elastin (i). Error bars, mean ± s.d. (*p < 0.05, **p < 0.01), (n = 3).
