Figure 6
From: Evaluation of a Miniaturized Biologically Vascularized Scaffold in vitro and in vivo
mBioVaSc as platform technology for tissue maintenance in vivo. Metabolic highly active liver-like organoids have been generated in vitro, transferred into the luminal part of the mBioVaSc and implanted for 3 (a–e), 7 (a’–e’) and 30 (a”–e”) days. Representation of the organoid-incorporated cell types via immunohistochemical staining for cell type specific markers - CK18 (a-a”), CD31 (b-b”) and CD90 (c-c”) - used for the employed cell types – hepatocytes, endothelial cells and mesenchymal stem cells, respectively. Highlighting proliferative properties of the cells by Ki67 (d-d”) staining, whereas activated cleaved Caspase3 (e-e”) was used as a marker for apoptosis. Scale bar, 100 µm.
