Figure 2
IL-33-stimulated ERK and NF-κB signalling is ST2-dependent and enhanced by IgE-mediated sensitisation of PDMCs and BMMCs. (a) PDMCs sensitized with murine anti-DNP IgE (0.5 μg/ml) overnight (IgE) or not (PBS) were cultured with IL-33 (10 ng/ml) for the indicated times and expression of activated (dually phosphorylated) and total ERK1 (p44) and ERK2 (p42) MAPK and IκBα analysed by Western blotting. β-actin was used as a loading control. (b) Wild type (WT) and ST2 −/− BMMCs sensitised with IgE were stimulated with IL-33 (10 ng/ml) for the indicated times and expression of activated (dually phosphorylated) and total ERK1 (p44) and ERK2 (p42) MAPK and IκBα analysed by Western blotting. β-actin was used as a loading control.
