Figure 6
PAM colocalizes with apical actin and cilia in tracheal epithelial cells. Transmission electron micrographs of wildtype murine tracheal epithelium revealed multiciliated cells with a dense array of microvilli extending from the apical surface (A,B). Numerous membranous structures were readily detected near the basal bodies, which were docked at the plasma membrane (arrows and inset in B). Immunogold electron microscopy indicated that PAM was present in the peri-basal body region of tracheal cells (C), and was closely associated with cilia (D,E). Immunostaining of airway epithelial cells with Bodipy-phalloidin (red) and PAM antibody (green) (F); PAM (green) and ezrin (red) (G); and FITC-phalloidin (green) and ezrin (red) (H). The inset in (F) shows the ciliary PAM signal. Note that PAM staining in these cells was previously shown to be abolished by treatment with the antigenic peptide13. Bars = 2 μm (A), 500 nm (B–D), 250 nm (E), and 5 μm (F–H).
