Table 1 Genotypes obtained from pammbg5+/− × pammbg5+/− crosses.

From: Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme

Clutch Number

Stage Analyzed

Genotype

n (%)

χ2 Test for Expected Mendelian Ratios

1

48 hpf

+/+

+/−

−/−

24 (23%)

54 (51%)

28 (26%)

0.34 (P < 0.9 but >0.5)

2

72 hpf

+/+

+/−

−/−

26 (27%)

54 (57%)

16 (17%)

3.57 (P < 0.5 but >0.1)

3

5 dpf

+/+

+/−

−/−

21 (22%)

54 (57%)

19 (20%)

2.17 (P < 0.5 but >0.1)

4

7 dpf

+/+

+/−

−/−

17 (24%)

40 (57%)

13 (19%)

3.86 (P < 0.5 but >0.1)

5

7 dpf

+/+

+/−

−/−

8 (27%)

17 (57%)

5 (17%)

1.13 (P < 0.9 but >0.5)

  1. The genotypes of five clutches of embryos from pammbg5+/− × pammbg5+/− crosses were determined. Wildtype, heterozygous and homozygous mutant siblings were obtained at approximately the expected Mendelian ratios for all stages examined from 48 hpf to 7 dpf (days post-fertilization); for the five clutches combined, χ2 = 5.58, DF = 2, P < 0.1 but >0.05. Percentages do not always sum to 100% due to rounding.
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