Figure 3

Effects of MPA + hMG on ovarian follicle development. Histologic sections were prepared from the ovaries of each group 24 hours after MPA or hMG treatment and stained with H&E, a detailed histopathological examination was performed under a light microscope noting each of the ovarian components. (A) Normal ovary of a control mouse. (B) The ovary with decreasing antral follicle and corpus luteum after MPA treatment. (C) The ovary with increasing corpus luteum after hMG treatment. (D) The ovary with increasing antral follicle and decreasing corpus luteum after MPA + hMG treatment. (E) The percentage of primordial follicle in four groups after 5 days of treatment. (F) The percentage of secondary follicle in four groups after 5 days of treatment. (G) The percentage of antral follicle in four groups after 5 days of treatment. (H) The percentage of corpus luteum in four groups after 5 days of treatment. Black arrows represent secondary follicle, yellow arrows represent antral follicle, green arrows represent corpus luteum. *Represents MPA + hMG group vs control group, MPA group or hMG group. *P < 0.05, ***P < 0.001.