Figure 5

GSH production and DCW of combinatorial strains with the modulation of both the Cys biosynthetic pathway and PDI. (A) GSH production of combinatorial strains based on the evolved strain W303-1b/FGPPT-8–12 and the engineered strain W303-1b/FGPPT. (B) DCW (g/L). (C) GSH content (%). The values are presented as the means, and the error bars show the SD (n = 3). ‘Control’ represents W303-1b/FGPPT-8–12/ΔSTR3 and W303-1b/FGPPT/ΔSTR3, respectively.