Figure 6
From: Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice
In vitro analysis of surface expression of podocalyxin and Rho-GTPase activity using a podocyte cell line. ERM protein expression levels in E11 cells were investigated by immunoblotting. Knockdown of ezrin was performed by siRNA. The siRNA targeting ezrin gene effectively down-regulated expression of ezrin in E11 cells (a). A surface biotinylation assay was performed using negative control siRNA- and ezrin siRNA-treated E11 cells. Surface expression of podocalyxin was examined and no contamination of cytosolic proteins was identified by immunoblotting for GAPDH. Densitometry analysis was performed (n = 5 for each group) (b). RhoA and Rac1 activity in siRNA-treated E11 cells was investigated by Rho-GTPase activity assay (n = 5 for each group). *P < 0.05, vs. Negative siRNA-treated E11 cells (c).
