Figure 2 | Scientific Reports

Figure 2

From: Biotic and environmental stress induces nitration and changes in structure and function of the sea urchin major yolk protein toposome

Figure 2

Toposome nitration in the gonads of sea urchins. C, control (pre-bloom animals); T0, animals collected during the bloom; T1, after stabulation for 56 days; T2, after stabulation for 92 days. (a) Representative experiment showing the western blot analyzed with anti-nitrotyrosine antibody. (b) Histogram showing densitometric analysis of immunopositive toposome respect to control and expressed as fold average increase value ±SE (One-way ANOVA, Dunnet’s post hoc test). The ratio between band intensity values of exposed and control animals was assumed as 1. The bands were quantified by Java Image software. Asterisks indicate significant differences compared to the control (*P < 0.05, **P < 0.01) N = 3.

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