Figure 6 | Scientific Reports

Figure 6

From: The evolution of insecticide resistance in the brown planthopper (Nilaparvata lugens Stål) of China in the period 2012–2016

Figure 6

Transgenic expression of CYP6ER1 (A,C,E and G) and CYP6AY1 (B,D,F and H) in D. melanogaster and their effects on imidacloprid, thiamethoxam and buprofezin resistance. (A and B) The expressions of CYP6ER1 and CYP6AY1 were confirmed by RT-PCR in two control lines and transgenic line. Three biological replicates of Da-GAL4, flies with genetic background correspond to Da-GAL4; Three biological replicates of UAS-CYP6ER1 (A) or UAS-CYPAY1 (B), flies not expressing the CYP6ER1 or CYP6AY1; Three biological replicates of Da > CYP6ER1 (A) or Da > CYP6AY1 (B), transgenic flies expressing the CYP6ER1 or CYP6AY1. (C,D,E,F,G and H). The comparison between survival rates of two control lines and transgenic line exposed to 1 mg/ml imidacloprid (C and D), thiamethoxam (E and F) and 400 mg/ml buprofezin (G and H). The data shown are the mean ± s.e.m. (n = 3). **P < 0.01, ***P < 0.001 (Chi-squared Test), ns (no significant).

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