Figure 7

Knockdown in the expression of CYP6ER1 in N. lugens field-resistance strain reduced its resistance to thiamethoxam and buprofezin. (A and C) The mRNA levels of CYP6ER1 were quantified by qRT-PCR at three days after dsRNA injection in FQ2016 (A) or buprofezin-resistance (C) strains, respectively. The data shown are mean + SEM (n = 3). Statistical significance of the gene expression between two samples was calculated using Student’s t test. *P < 0.05, **P < 0.01 and ***P < 0.001. (B and D) Effects on the mortality rate after injection of dsGFP or dsCYP6ER1 and followed by application of thiamethoxam (5 mg/L) (B) or buprofezin (50 mg/L) (D).