Figure 5

Flow cytometric analysis of relative DCF oxidation. (A) Flow charts of NHCs (I) after 72 h incubation with IC50 doses of DDC (II), uncoated NPs (III) and coated NPs (IV) as well as Hepa (V) after 72 h treatment with IC50 doses of DDC (VI), uncoated NPs (VII) and coated NPs (VIII). (B) The fold change in DCF fluorescence after treatment with DDC and its NPs relative to untreated NHCs and Hepa (* and §represent significance at p < 0.01 when compared to DDC- treated cells and uncoated NPs-treated cells, respectively).