Figure 2
From: Stimulation of alpha2-adrenergic receptors impairs influenza virus infection
Inhibitory effect of clonidine on influenza virus replication. (A,B) Effect of clonidine on influenza virus replication. AX4/PB2 cells were treated with the indicated concentration of clonidine 1 hour before viral infection. In the presence of clonidine, the AX4/PB2 cells were infected with WSN/PB2-Rluc virus. After incubation in the presence of clonidine, virus titers were determined by using plaque assays (A), and NA levels in the supernatant were measured with the NA-Star kit (B). (C) Effect of clonidine on the replication kinetics of influenza viruses. AX4/PB2 cells were treated with 1 µM clonidine (Red) or DMSO (Black), and infected with WSN/PB2-Rluc virus at an MOI of 0.025. The amount of virus was determined at the indicated time points by measuring the NA levels in the supernatant. Data are shown as means ± SD of triplicate assay wells (A and C) or means ± SEM of three independent experiments (B). *P < 0.005.
