Figure 1
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
Experimental strategy used for the stable transfection of B. bigemina and B. bovis cell lines. (a) Schematic representation of the target ef-1α locus of B. bigemina in B. bigemina parasites (bigemina-big-ef-egfp-bsd). (b) Schematic representation of the stable transfection plasmid pbig-ef-egfp-bsd used for electroporation. (c) Schematic representation of the target ef-1α locus of B. bigemina in B. bovis parasites (bovis-big-ef-egfp-bsd). BgIII: restriction enzyme.
