Figure 3
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
In vitro growth curve of B. bigemina and B. bovis parasites up to 72 hr. (a) B. bigemina growth curve. (b) B. bovis growth curve. Tbg + bsd: bigemina-big-ef-egfp-bsd parasites in the presence of blasticidin; Tbg-bsd: bigemina-big-ef-egfp-bsd parasites in the absence of blasticidin; wt Bbg + bsd: B. bigemina wild-type parasites in the presence of blasticidin; wt Bbg-bsd: B. bigemina wild-type parasites in the absence of blasticidin; Tbo + bsd: bovis-big-ef-egfp-bsd parasites in the presence of blasticidin; Tbo-bsd: bovis-big-ef-egfp-bsd parasites in the absence of blasticidin; wt Bbo + bsd: B. bovis wild-type parasites in the presence of blasticidin; wt Bbo-bsd: B. bovis wild-type parasites in the absence of blasticidin. Normalized PPE values (Y axis) obtained from Babesia spp. in the presence or absence of blasticidin (X axis). Media was used as negative controls for no inhibition. Error bars indicate standard deviations for each sample tested from triplicate culture. Data were compared using ANOVA analysis. *Represents P < 0.05 indicating a statistically significant difference between groups.
