Figure 4
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
Southern blot analysis using dig-labeled probes against: (a) B. bigemina rap-1a; (b) B. bovis msa-1; (c) egfp and (d) ef-1α. 1: wild-type B. bigemina gDNA digested with BgIII; 2: wild-type B. bigemina gDNA undigested; 3: bigemina-big-ef-egfp-bsd digested with BgIII; 4: bigemina-big-ef-egfp-bsd undigested; DM: Dig labeled DNA marker II; Pr-: pBS promoterless control plasmid; C-: pbig-ef-egfp-bsd plasmid control; 5: wild-type B. bovis gDNA digested with BgIII; 6: wild-type B. bovis gDNA undigested; 7: bovis-big-ef-egfp-bsd digested with BgIII; 8: bovis-big-ef-egfp-bsd undigested. Vertical black stripes on the blot indicate cropping of the blot. A full image of the original blot can be seen in Supplementary Info section.
