Figure 5
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
PCR integration analysis in B. bigemina using two different set of primers. (a) egfp-Fwd and ef1α-Rev. (b) egfp-Fwd and egfp-Rev. Line 1: wild-type B. bigemina gDNA; Line 2: bigemina-big-ef-egfp-bsd; C-: pbig-ef-egfp-bsd plasmid control; MW: molecular size ladder in bp, 1 Kb Plus DNA ladder.
