Figure 6
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
PCR integration analysis in B. bovis culture using two different sets of primers. (a) egfp-Fwd and Bbov-UpS-efB-Rev. (b) rap-1a-Fwd and rap-1a-Rev. Line 1: bovis-big-ef-egfp-bsd gDNA; line 2: wild-type gDNA B. bovis; MW: molecular size ladder in bp, 1 Kb Plus DNA ladder. (c) Alignments among the regions of insertion of the transfected genes into bigemina-big-ef-egfp-bsd and bovis-big-ef-egfp-bsd parasites. Vertical black stripes on the blot indicate where the image was cropped. Full image of the original agar electrophoresis gel can be seen in Supplementary Info section.
