Figure 7
From: Stable transformation of Babesia bigemina and Babesia bovis using a single transfection plasmid
Immunoblot analysis. (1) wild-type B. bigemina. (2) bigemina-big-ef-egfp-bsd. (3) wild-type B. bovis. (4) bovis-big-ef-egfp-bsd. (5) Uninfected bovine RBC. Samples were incubated with antibodies: (a) pre-immune mouse serum; (b) anti-B. bigemina rap-1 MAb. (c) anti-B. bovis rap-1 MAb. (d) anti-GFP MAb. (M) molecular size ladder in kDa indicated by arrows. Vertical black stripes on the blot indicate cropping of the blot. A full image of the original blot can be seen in Supplementary Info section.
