Table 3 SEC-SAXS data.

From: Structural modelling of the DNAJB6 oligomeric chaperone shows a peptide-binding cleft lined with conserved S/T-residues at the dimer interface

Sample

a R g , nm

a D max , nm

a V p , nm 3

b MW p , kDa

b MW a , kDa

c Subunits

DNAJB640

8.73 ± 0.16

29.6

2368

1480

1450

55

DNAJB641

8.50 ± 0.35

29.4

2114

1321

1240

49

DNAJB642

7.70 ± 0.2

27.5

1849

1156

1090

43

DNAJB643

7.43 ± 0.11

26.6

1669

1043

979

39

DNAJB644

6.72 ± 0.25

22.5

1523

952

856

35

DNAJB645

6.88 ± 0.19

22.2

1470

919

838

34

  1. aRg, Radius of gyration, Dmax, maximum size, Vp, Porod volume, calculated as described in Methods.
  2. bMWp, MWa, mass estimates calculated by dividing Vp by 1.6 and by the DAMMIF model excluded volume by 2, respectively.
  3. cSubunits, number of subunits fitting the average MWp calculated with mass of the DNAJB6 26.9 kDa.
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