Figure 3
From: Enterobacter bugandensis: a novel enterobacterial species associated with severe clinical infection
Whole genome transcriptome analysis using an RNA-Seq approach. (A) Experimental design of the RNA-seq approach. Schematic illustration of the steps that are employed during the sequencing procedure. (B) Differential expression analysis of normalized reads. After normalization, the sequence reads were analyzed by using the DESeq2, baySeq and edgeR packages. Individual packages were run independently and genes that were common according to all three different analyses were considered as differentially regulated. (C) Correlation between the average read counts of individual genes regulated in both LB and serum. Red and green dots represent the individual genes that are up and down regulated respectively, whereas the black dots represent the genes that are not differentially regulated. Each dot represents the paired coordinate value between the mean numbers of reads of individual genes obtained from experiments from three biological repeats. The correlation coefficient value was determined by using the approach ‘Goodness of Fit’ of GraphPad Prism V5.
