Figure 1
From: Tunable Ampere phase plate for low dose imaging of biomolecular complexes

(a) Secondary electron image of the TAPP device recorded before connecting it to a counter-electrode. (b,c) Bright-field images of the device viewed in the specimen plane in a TEM at tilt angles of 70° and 0° with respect to the optic axis of the microscope, respectively. The counter-electrode, which is movable in the present setup, is visible on the right of each image. (d–f) 8× amplified phase images of the vacuum region around the phase plate recorded using off-axis electron holography for currents through the device of (d) 0, (e) 2 and (f) 4 mA. (g–i) Simulated 8× amplified phase images for currents of (g) 0, (h) 2 and (i) 4 mA, including the influence of the perturbed reference wave.