Figure 2
From: Tunable Ampere phase plate for low dose imaging of biomolecular complexes
(a)Molecular structure of the NCP. (b) Amplitude and (c) phase of the electron wave transmitted by the NCP at 200 kV; the images are density-plotted over their full ranges: 0.770–0.790 for the amplitude and 1.59–1.71 rad for the phase. (d–f) Simulated out-of-focus Fresnel images for defocus values of (d) 0.05, (e) 0.5 and (f) 1.0 µm. (g–i) Simulated TAPP images for currents of (g) 0.2, (h) 0.6 and (i) 3.0 mA. The image contrast C = (Imax − Imin)/(Imax + Imin) is specified at the top right corner of each simulation. (j) and (k) show how typical levels of noise would affect the object visibility for the out-of-focus and TAPP images.
