Figure 2
Reproducibility of microarray results evaluated by coefficient of variation. Each peptide was spotted 2–3 times on a chip for intra-assay readings, and each array was tested 2–3 times for inter-assay readings. The overall average of 4–9 intra- and inter-assay signals were used to assess functional peptide antigens in the microarray as shown in Fig. 1. (A) All individual readings are plotted versus their corresponding averages, and the 0.2 cut-off is shown that separates weak signals with high variance from strong signals with low variance in intra- and inter-assays. (B,C) The means in intra- and inter-assays are plotted versus their corresponding coefficient of variation (CV).
