Figure 6
Detection of human exposure to C. trachomatis and C. pneumoniae. (A) C. trachomatis-specific antibody detection by use of the strongly reactive outer membrane protein A (OmpA), PmpD, and CT529 peptides. Three strain-variant peptides of OmpA variable domain IV, two peptides of two separate regions from polymorphic membrane protein D (PmpD), and one peptide of protein CT529 (CT_529 locus of C. trachomatis D/UW-3/Cx) were used to determine C. trachomatis-specific antibodies from 43 serum samples. Separately, in an ELISA with recombinant C. trachomatis OmpA antigen, anti-C. trachomatis antibody levels of these 43 sera were determined, as displayed below the abscissa where 4, 3, 2, 1, or 0 indicates very strong, strong, moderate, weak, or absent ELISA signal. ELISA signals 4-3 are classified as positive, 2 as borderline, and 1 (above background) and 0 (background signal) as negative. (B) C. pneumoniae- and remaining Chlamydia spp.-specific antibody detection. The strongest microarray signals with an IncA peptide and a PmpD peptide of C. pneumoniae are shown. Among the remaining 9 Chlamydia spp., three C. avium and C. gallinacea peptides produced signals with three individual sera. All peptides of the remaining 7 Chlamydia spp. produced <0.1 signals in the microarray and are not shown. In a C. pneumoniae ELISA with an antigen of complexes of outer membrane proteins (COMC), antibody levels of these 43 sera against C. pneumoniae were determined. (C) Correlation between C. trachomatis OmpA ELISA signals and C. trachomatis microarray signals. (D) Correlation between C. pneumoniae COMC ELISA signals and C. pneumoniae microarray signals. (E) Correlation between C. trachomatis microarray signals and C. pneumoniae microarray signals.
