Figure 1

Maturation stability of hMoDCs and hiPSDCs. (a) Characterization of human iPSCs. Alkaline phosphatase staining and fluorescent staining with undifferentiated markers showed pluripotency of human iPSCs. Scale bars = 80 μm. (b) The schematic diagram of differentiation protocol for hiPSDCs. Scale bars = 80 μm (Before Day 16). Scale bars = 20 μm (After Day 21). (c) Morphology of mature hMoDCs on day seven and mature hiPSDCs on day 23. Scale bars = 20 μm. (d) Surface phenotypes of hMoDCs and hiPSDCs. Histograms show the staining results of specific antibodies (black) and isotype-matched controls (thin lines). (e) Secretion of human IFN-γ and human IL-12 (p70) from hMoDCs and hiPSDCs. Data represent the mean ± SD (three donors for each group). *Significantly higher than the immature DCs. (P < 0.01) **No significant differences between the mature hMoDCs and mature hiPSDCs (P > 0.05). (f) Migration capacity of hMoDCs and hiPSDCs, which were examined by the expression of CCR7, using flow cytometry. Histograms show staining results of specific antibodies (black) and isotype-matched controls (thin lines). (g) Chemotactic assay in vitro. Data represent the mean ± SD (three donors for each group). Almost 30% of the mature hiPSDCs migrated in response to macrophage inflammatory protein 3β chemokine in transwells, while less than 5% of the immature hiPSDCs did so. *Significantly higher than the immature DCs (P < 0.01). A similar migratory capacity was observed in hMoDCs and hiPSDCs.