Figure 3 | Scientific Reports

Figure 3

From: Cancer Vaccine Therapy Using Carcinoembryonic Antigen - expressing Dendritic Cells generated from Induced Pluripotent Stem Cells

Figure 3

Cytotoxic activity of cytotoxic T cells generated from hiPSDCs-CEA. CD8+ cytotoxic T cells were sorted using an autoMACS Pro Separator after three cycles of stimulation of autologous peripheral blood mononuclear cells by genetically modified DCs, and the cytotoxic activity was tested using a 4-h 51Cr release assay. Cytotoxic T cells induced by hiPSDCs-CEA from each of the three HLA-A24-positive donors exhibited cytotoxic activity against LCLs-CEA and LCLs-CEA652, whereas they exhibited no cytotoxicity against LCLs-LacZ. Cytotoxic T cells induced by hiPSDCs-LacZ from each HLA-A24-positive donor exhibited no cytotoxic activity against LCLs-CEA, LCLs-CEA652, or LCLs-LacZ.

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