Figure 5

(A) Real-time quantitative PCR demonstrated that transfection of cardiomyocytes with miR-19a-3p mimics increased the expression of miR-19a-3p level, while miR-19a-3p inhibitor reduced the expression of miR-19a-3p level, but SFI increased the expression of miR-19a-3p level (**P < 0.01, n = 3 for each group). ^△△p < 0.01 vs. control, ^**p < 0.01 vs.PE + NC. ^##p < 0.01 vs. PE+ miR-19a-3p mimics. ^&&p < 0.01 vs. PE+ miR-19a-3p inhibitor. (B) MEF2A mRNA level was significantly down-regulated in miR-19a-3p mimic group, and significantly up-regulated in miR-19a-3p inhibitor group, but SFI increased the expression of MEF2A mRNA level (**P < 0.01, n = 3 for each group). ^△△p < 0.01 vs. control, ^**p < 0.01 vs.PE + NC. ^## p < 0.01 vs.PE+ miR-19a-3p mimics. ^&&p < 0.01 vs. PE+ miR-19a-3p inhibitor. (C and D) In the medium supplemented with PE, over-expression of miR-19a-3p reduced the cell size as measured by cell surface area (100 mM). Lower-expression of miR-19a-3p increased the cell size (100 mM). SFI reduced the cell size either transfected with miR-19a-3p mimics or inhibitor. n = 6, ^△△p < 0.01 vs. control, ^**p < 0.01 vs.PE + NC. ^##p < 0.01 vs. PE+ miR-19a-3p mimics. ^&&p < 0.01 vs. PE+ miR-19a-3p inhibitor.