Figure 1

Palmitate induced lipid accumulation and suppressed lipolysis in HepG2 cells. (A and B) Dose–response relationship between palmitate and lipid accumulation. (A) HepG2 cells were stained with Oil Red O to visualize the intracellular lipid contents (original magnification, ×100). (B) Lipid accumulation was quantified by the absorbance value of the extracted Oil Red O dye at 500 nm. The data from each group are presented as the means ± SEs (n = 3). **P < 0.01. (C) Dose–response relationship between palmitate and inhibition of lipolysis. Lipolysis was assessed by the release of glycerol into the media. The data from each group are presented as the means ± SEs (n = 3). **P < 0.01.