Figure 3

Nur77 suppressed the palmitate-induced expression of PPARγ and its target genes in HepG2 cells. (A) HepG2 cells were transfected with pReceiver-M98 or pReceiver-M98-Nur77, and Nur77 expression was measured through a Western blot analysis. The data from each group are presented as the means ± SEs (n = 3). **P < 0.01. (B) Nur77 overexpression decreased the palmitate-induced mRNA expression of PPARγ and its target genes (G0S2, GPR81, GPR109A, and Adipoq). mRNA expression was measured by a quantitative PCR analysis. The data from each group are presented as the means ± SEs (n = 3). *P < 0.05, **P < 0.01. (C) Nur77 overexpression decreased the palmitate-induced protein expression of PPARγ and G0S2. Protein expression was measured through a Western blot analysis. The data from each group are presented as the means ± SEs (n = 3). **P < 0.01.