Figure 4
Microgliosis in Psmc1fl/fl;CaMKIIα-Cre forebrain. (A) Representative horizontal brain sections immunostained with Iba1. No significant differences were evident in control cortices; therefore the control image is representative of all ages. Insets show the region of cortex shown (LHS; oval) and higher magnification images of the pathology (RHS; white boxes). Scale bar = 500 (LHS; cortex) and 200 (RHS; hippocampus) µm. Quantitation of microglia in control and Psmc1fl/fl;CaMKIIα-Cre cortices (B) and hippocampi (C) using the percentage area stained by Iba1 and microglial soma size. Left and right cortices or hippocampi of n = 3 mice at each age were analysed; mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 by unpaired Students t-test.
