Figure 5 | Scientific Reports

Figure 5

From: Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons

Figure 5

Increased GDH and GPBB in Psmc1fl/fl;CaMKIIα-Cre forebrain. (A) Representative immunoblots and quantification of GDH and GPBB in mitochondria and cytosol respectively purified from control and Psmc1fl/fl;CaMKIIα-Cre cortices at 6 weeks-old. Pyruvate dehydrogenase E1 component subunit alpha (PDHE1α; mitochondria) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cytosol) were used as a loading controls. Images were cropped from those shown in Supplementary Figure 6. Mean ± SEM of n = 3 mice. *p < 0.05 and **p < 0.01 by unpaired Students t-test. (B) Immunostaining of cortical and hippocampal brain sections from control and Psmc1fl/fl;CaMKIIα-Cre mice at 6 weeks-old with GDH and GPBB. Asterisks and arrows indicate astrocytes and Lewy-like inclusions respectively. Scale bar = 50 µm.

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