Figure 7

Preliminary steps of metastasis in bioreactor. (A) Schematic representation of the set-up for observation of cell spread and intravasation. Bioreactor was developed by React4life S.r.l. (B) Electrospun membrane used to mimick blood vessel interface. The membranes were realized in polycaprolacton (PCL) by electrospinning technique. (C–F) Confocal slides of cell entrapped within the membrane (cell is in false colours).