Figure 2

Cellular response of mMSCs exposed to adipogenic differentiation under standard or hypothermal conditions. (a) Metabolic activity measured in AD-treated cultures maintained at 32 and 37 °C. (b) ORO staining of cells after 9 days of differentiation at 32 and 37 °C, and (c) changes in LD size distribution (mean diameter). Scale bars, 20 μm. (d) Changes in lipid content per well in cells differentiated at 32 and 37 °C. ***Comparison of the same treatments at different temperature; °°°Comparison of different treatments at the same temperature. Statistical significance was set at p < 0.05. (e) Proportion of lipid-containing cells after differentiation at 32 °C and 37 °C. Measurements (c and e) were done on 50 randomly selected micrographs per condition (n = 3 individual experiments). Data are shown as mean ± SEM. Statistical significance was set at p < 0.05.