Table 2 V565 specifically neutralizes human and cynomolgus monkey TNFα.

Assay	Ligand	Antibody	Functional Readout	Binding/Potency
ELISA^a	Human TNFα	V565	Inhibition of V565-human TNFα interaction	+++
ELISA^a	Cyno TNFα	V565	Inhibition of V565-human TNFα interaction	+++
ELISA^a	Rabbit TNFα	V565	Inhibition of V565-human TNFα interaction	—
ELISA^a	Murine TNFα	V565	Inhibition of V565-human TNFα interaction	—
ELISA^a	Human LTα	V565	Inhibition of V565-human TNFα interaction	—
L929 cells	Human TNFα	V565	Inhibition of TNFα-induced cell cytotoxicity	0.27 nM^b
L929 cells	Cyno TNFα	V565	Inhibition of TNFα-induced cell cytotoxicity	0.08 nM^b
L929 cells	Cyno TNFα	adalimumab	Inhibition of TNFα-induced cell cytotoxicity	0.05 nM^b

^aThe ability of human TNFα and TNFα from other species to bind V565 was determined using an adalimumab-TNFα binding competition ELISA format as described in the methods section.
^bIC₅₀ concentration of V565 or adalimumab required to achieve 50% inhibition of maximal TNFα-induced response.

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