Figure 1
From: Detection of aryl hydrocarbon receptor agonists in human samples
AHR agonistic activity in human sera is influenced by the species of the cell line used in the reporter assay. (a) Human embryonic kidney cells (HEK293) were transfected with pGud-Luc1.1 and pTK-Renilla. One day after transfection, cells were incubated with human serum from healthy controls (HC) or MS patients (MS) and luciferase activity was assessed after 24 hours. Control cultures to which no serum was added were used to determine baseline activity. (b,c) Luciferase assay performed as in (a) using human HepG2 (b) or mouse HEPA1 (c) cell lines. (d) A stable AHR reporter mouse H1G1.1C3-Luc cell line was used to measure AHR ligand activities 24 hours after incubation with HC or MS serum. FACS analysis of mean fluorescence intensity (MFI) for reporter expression. *P < 0.05, **P < 0.01 and ***P < 0.001 by one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test.
