Figure 5

The wild type (WT) and mutated (I74T) HspA1A variant bind similarly to ATP. Representative Isothermal Titration Calorimetry (ITC) assays using purified recombinant HspA1A proteins and ATP. (a,b) Represent the ITC raw data for 20 automatic injections of ATP into the sample cell containing either WT or mutated HspA1A. (c,d) Represent ITC binding curves obtained for the interaction between ATP and HspA1A. The data shown are representative of three independent experiments.