Figure 6

The mutated HspA1A variants have similar localization as compared to the WT protein. Quantification of the corrected total cell fluorescence (CTCF) as a ratio between the total GFP-HspA1A fluorescence of particular intracellular compartment [(a) nucleus, (b) mitochondria, (c) lysosome, and (d) plasma membrane] and the rest of the cell. White bars indicate cells that maintained at 37 °C and grey bars indicate cells that were stressed at 42 °C for 60 min. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software (http://shiny.chemgrid.org/boxplotr/); whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; crosses represent sample means. The experiment was repeated three times and in total 15 cells were used to generate each boxplot. Star (*) denotes statistical significance. The p-values of the student Holm test were: S16Y-37 vs 42 = 0.04; F592S-37 vs 42 = 0.01 and WT-37 vs 42 = 0.01.