Table 1 BiFC plasmid reagents constructed in this study*.

From: MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI)

Constructs tagged at N-term

Constructs tagged at C-term

pACEMam1-VenNt-GOI

pACEMam1-GOI-VenNt

pACEMam1-VenCt-GOI

pACEMam1-GOI-VenCt

pACEMam1-mCherry-GOI

pACEMam1-GOI-mCherry

pACEMam1-eGFP-GOI

pACEMam1-GOI-eGFP

pMDCP-VenNt-GOI

pMDCP-GOI-VenNt

pMDCP-VenCt-GOI

pMDCP-GOI-VenCt

pMDCP-eGFP-GOI

pMDCP-GOI-eGFP

  1. *GOI,gene of interest; Ven, Venus fluorescent protein; Nt, N-terminal fragment, Ct, C-terminal fragment, pACEMAM1 and pMDC, plasmid reagents from MultiMam system10 (http://www.embl.fr/multibac/multiexpression_technologies/multimam).
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