Figure 2 | Scientific Reports

Figure 2

From: Dynamic analysis of the interactions between Si/SiO2 quantum dots and biomolecules for improving applications based on nano-bio interfaces

Figure 2

Time evolution of Si/SiO2 QDs protein corona in different media. (a) Representation of a BSA calibration curve obtained in the range of 0–20 µg by SDS-PAGE. (b) QDs were incubated with 20 µg/ml BSA and the amount of adsorbed BSA was calculated as ng after extrapolating the band intensity on the BSA standard curve and expressed also as % of the initial amount of suspended BSA. (c) Representation of a MEM + 10% FBS calibration curve obtained for protein levels in the range of 0.75–90 µg by SDS-PAGE. (d) QDs were incubated with MEM supplemented with 10% FBS and the total amount of adsorbed proteins and the level of adsorbed BSA were quantified based on the MEM + 10% FBS calibration curve and expressed as µg. Results are expressed mean ± SD (n = 3).

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