Table 1 Dynamic adsorption of proteins by Si/SiO2 QDs in different media expressed in percentages normalized to the initial protein content.
Media | Protein | Control | Time of incubation with QDs | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Initial protein amount | % of all protein content | 5 min | 15 min | 30 min | 1 h | 3 h | 5 h | 8 h | 24 h | ||
BSA | BSA adsorbed on QDs (%) | 20 µg | 100% | 2.91% | 4.02% | 5.72% | 6.27% | 4.93% | 4.02% | 3.74% | 3.15% |
MEM + 10% FBS | Protein adsorbed (% of all protein content) | 6.75 mg | 100% | 0.60% | 0.61% | 0.63% | 0.62% | 0.59% | 0.58% | 0.57% | 0.41% |
BSA adsorbed on QDs (% of initial BSA amount) | 3 mg | 100% | 0.253% | 0.266% | 0.273% | 0.276% | 0.273% | 0.272% | 0.269% | 0.254% | |
BSA adsorbed on QDs (% of all proteins adsorbed) | 3 mg | 44% | 18.60% | 19.08% | 19.18% | 19.51% | 20.54% | 20.80% | 20.84% | 27.17% | |