Table 3 Metrics of high-quality single nucleotide variants (SNVs) detected across Everglades Virus EVG3–95 genome via Illumina sequencing of virus-positive mosquito pool sample 4.1. NT Position (column 1) based on NCBI accession #KR260737. All SNV presented here were 100% penetrant at indicated reference position. 10 out of 16 SNVs (~62%) were detected via MinION reads.

From: Unbiased Strain-Typing of Arbovirus Directly from Mosquitoes Using Nanopore Sequencing: A Field-forward Biosurveillance Protocol

NT Position

Reference

Allele

Illumina Coverage

Detected via Nanopore?

2926

C

T

43

NO

3268

C

T

53

YES

3365

C

T

49

YES

4063

G

A

33

NO

5521

T

C

54

YES

6718

C

T

36

NO

7534

A

C

33

YES

7584

G

A

30

YES

7900

G

A

52

YES

8094

T

C

71

YES

9147

C

T

49

NO

10032

G

A

85

YES

10317

G

A

32

NO

10425

C

T

57

NO

10539

T

C

51

YES

10731

T

C

128

YES

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